INORGANIC CATIONS IN THE CELL NUCLEUS
نویسندگان
چکیده
منابع مشابه
Inorganic Cations in the Cell Nucleus
Earlier reports indicated the presence of significant amounts of inorganic salts in the nucleus. In the present study the possibility that this might be related to the transcription process was tested on seminiferous epithelium of the adult mouse, using potassium pyroantimonate as a fixative. The results indicated that a correlation exists between the inorganic cations comprising the pyroantimo...
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For localization of pyroantimonate-precipitable cations, rat kidney was fixed by perfusion with a saturated aqueous solution of potassium pyroantimonate (pH about 9.2, without addition of any conventional fixative). A remarkably good preservation of the tissue and cell morphology was obtained as well as a consistent and reproducible localization of the insoluble antimonate salts of magnesium, c...
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Inorganic cations have been localized in several animal and plant tissues by means of the pyroantimonate fixation procedure (1-3) . We demonstrated that an aqueous solution of potassium pyroantimonate enters the cell without disruption of cell membranes and produces electron-opaque cation-antimonate precipitates in a constant and reproducible manner. The results obtained in mouse testis (3) wer...
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0.3-0.5 M MgCl(2) was used to disassemble nuclei and to isolate by a single centrifugation in less than 3 hr a nuclear envelope fraction in 55-60% yield as assessed by phospholipid recovery. Its gross chemical composition was determined and its morphology was studied electron microscopically by sectioning, freeze etching, and negative staining procedures.
متن کاملThe Distribution of Inorganic Cations in Mouse Testis
For localization of pyroantimonate-precipitable cations, mouse testes were fixed with a saturated aqueous solution of potassium pyroantimonate (pH about 9.2, without addition of any conventional fixative), hardened with formaldehyde, and postosmicated. A good preservation of the cell membranes and over-all cell morphology is obtained as well as a consistent and reproducible localization of the ...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1972
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.53.2.483